Plant tissue culture refers to a set of techniques employed to cultivate plant cells, tissues, or organs under sterile conditions on a nutrient culture medium of known composition. These techniques are frequently utilized for the commercial production of plants. Plant tissue culture methods find applications in various areas such as the production of ornamental shrubs and slow-growing woody trees, generating haploid plants through the cultivation of anthers, pollen grains, and embryos, as well as protoplast production of secondary metabolites. Plants for which protocols have been established in the Biotechnology Department of Behyaar Co. include Paulownia, Bell Pepper, and Periwinkle. Additionally, preliminary studies have been conducted on Roses, Damask rose, and Pear.